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F180 Inverted Phase Contrast Fluorescent Semiapochromat Biological Objective

F180 Inverted Phase Contrast Fluorescent Semiapochromat Biological Objective

The F180 Inverted Phase Contrast Fluorescent Semiapochromat Biological Objective is a series of high-performance objectives designed for inverted microscopes in life science and biomedical research. It supports phase contrast and fluorescence imaging with semiapochromat correction, offering consistent resolution and contrast across multiple magnifications. The series includes 4X, 10X, 20X, 40X, and 60X models with numerical apertures from 0.15 to 0.75, working distances from 2 mm to 18 mm, and a field number of 25 mm. Designed for inverted systems, it enables clear observation of live cells, tissue cultures, and fixed specimens under both phase contrast and fluorescence illumination.

  • Semiapochromat correction reduces chromatic and spherical aberration for improved image clarity across multiple wavelengths
  • Inverted design allows direct observation of live cell cultures and specimens on culture dishes or plates
  • Phase contrast and fluorescence compatibility enables dual-mode imaging without objective change
  • Consistent 25 mm field number ensures uniform field of view across all magnifications
  • Parfocal length of 45 mm (inverted) or 60 mm (upright) ensures minimal refocusing when switching between objectives
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Technical features

  • Inverted fluorescent semiapochromat biological objective series with 4X, 10X, 20X, 40X, 60X magnifications
  • Numerical aperture range: 0.15 (4X) to 0.75 (60X)
  • Working distance: 18 mm (4X) to 2 mm (60X)
  • Field number: 25 mm across all models
  • Parfocal length: 45 mm for inverted configuration
  • Phase contrast and fluorescence compatible with standard filter cubes
  • Semiapochromat correction for improved chromatic and spherical aberration control
  • Designed for use with inverted microscopes in cell culture and live imaging

Who it is for

  • Biomedical research labs conducting live cell or fluorescence studies
  • Biopharmaceutical manufacturers requiring consistent imaging for cell culture monitoring
  • Clinical laboratories performing diagnostic cell analysis with phase contrast and fluorescence
  • University and academic research institutions using inverted microscopes for cell biology
  • Exporters sourcing standardized objectives for microscope kits or system integrations

Specifications

ModelF180 Inverted Phase Contrast Fluorescent Semiapochromat Biological Objective
Magnification4X, 10X, 20X, 40X, 60X
Numerical Aperture0.15 (4X), 0.3 (10X), 0.45 (20X), 0.6 (40X), 0.75 (60X)
Working Distance18 mm (4X), 10 mm (10X), 7.2 mm (20X), 3.7 mm (40X), 2 mm (60X)
Field Number25 mm
Parfocal Length45 mm (inverted), 60 mm (upright)
CorrectionSemiapochromat
CompatibilityInverted and upright microscopes with standard nosepiece
ApplicationPhase contrast, fluorescence, live cell imaging

Use guide

  • Select magnification and NA based on required resolution and specimen thickness
  • Use 4X–20X for live cell observation with larger working distance and field of view
  • Use 40X–60X for high-resolution imaging of cellular structures and subcellular details
  • Ensure the microscope stage and nosepiece are compatible with the 45 mm (inverted) or 60 mm (upright) parfocal length
  • Pair with appropriate filter cubes and light sources for fluorescence imaging

FAQ

What is the working distance for the 60X objective?
2 mm
Is this objective compatible with upright microscopes?
Yes, a version with 60 mm parfocal length is available for upright use.
What is the field number?
25 mm across all magnifications.
Can I use this objective for fluorescence imaging?
Yes, it is designed for fluorescence with semiapochromat correction and compatible with standard filter cubes.
What is the parfocal length?
45 mm for inverted configuration; 60 mm for upright configuration.

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